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. 2009 Feb 9;77(4):1700–1707. doi: 10.1128/IAI.01470-08

FIG. 2.

FIG. 2.

Generation of recombinant hybrid flagellins genetically fused to the CD4+ T-cell-specific gp43-derived P10 epitope. (A) Schematic representation of the recombinant flagellins after P10 in-frame insertion at FliC central hypervariable domain. The two recombinant flagellins carried the P10 peptide (FliCd-P10) or the P10 peptide with lysine residues on each side of the fusion site (FliCd-P10L). (B) Coomassie blue-stained 12% polyacrylamide gel loaded with flagellins extracted from different Salmonella strains. Lane 1, molecular mass markers (Fermentas); lane 2, FliCd flagellin extracted from S. Dublin strain SL5930 (with no insert); lane 3, FliCd-P10 flagellin extracted from S. Dublin strain SLP10; lane 4, FliCd-P10L flagellin extracted from S. Dublin strain SLP10L; lane 5, purified gp43. Each well was loaded with approximately 2 μg of purified flagellins or gp43.