TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| Salmonella Dublin | ||
| SL5928 | aroA his fliC::Tn10 | 24 |
| SL5930 | SL5928 with pLS408; mobile | 24 |
| SLP10 | SL5928 with pLSP10 | This study |
| SLP10L | SL5928 with pLSP10L | This study |
| P. brasiliensis | ||
| Pb18 | Highly virulent human isolate of P. brasiliensis | 35 |
| Plasmids | ||
| pLS408 | pUC19 derivative carrying the fliCd gene from S. Müenchen with 48-bp deletion in the hypervariable region of the FliC gene bla | 24 |
| pLSP10 | pLS408 derivative with a 45-bp insert encoding the P10 epitope | This study |
| pLSP10L | pLS408 derivative with a 57-bp insert encoding the P10 epitope with two flanking lysines | This study |