FIG. 4.

EMSA (A) or supershift assay (B) performed on nuclear proteins of SJPL and NPTr cells following an incubation (30 to 180 min) with A. pleuropneumoniae S4074 or no treatment for the control (Nil). For controls, proteins were incubated with specific oligonucleotides (S) and nonspecific oligonucleotides (NS). The probe alone was also loaded on the gel (P). For the supershift assay (B), proteins were incubated with p50 antibodies (p50), p65 antibodies (p65), or no antibodies (-). Arrows demonstrate the subunit band shifts (B).