TABLE 4.
A. pleuropneumoniae genes which are upregulated during adherence to SJPL cellsa
| Locus tag | Gene | Description | Fold change |
|---|---|---|---|
| Hypothetical/unclassified/unknown | |||
| APL_0568 | Hypothetical membrane protein | 3.083 | |
| APL_1459 | Hypothetical protein | 2.882 | |
| APL_1690 | Predicted periplasmic/secreted protein | 2.609 | |
| APL_1471 | Putative sugar transferase | 2.450 | |
| APL_1380 | Uncharacterized conserved protein | 2.310 | |
| APL_2002 | Hypothetical protein | 2.301 | |
| APL_0750 | MscS family protein | 2.181 | |
| APL_1575 | Hypothetical protein | 1.940 | |
| APL_1103 | Predicted inner membrane protein | 1.844 | |
| APL_1854 | pqiA | Uncharacterized paraquat-inducible protein A | 1.746 |
| APL_0217 | Hypothetical protein | 1.640 | |
| Biosynthesis of cofactors | |||
| APL_0776 | ispE | 4-Diphosphocytidyl-2C-methyl-d-erythritol 2-phosphate synthase | 2.913 |
| Energy metabolism | |||
| APL_1849 | lldD | l-Lactate dehydrogenase | 6.756 |
| APL_1820 | rpe | d-Ribulose-phosphate-3 epimerase | 6.687 |
| APL_1191 | namA | NADPH dehydrogenase | 4.524 |
| APL_1331 | hyaA | Hydrogenase 2 small chain precursor | 3.366 |
| APL_1685 | fucK | l-Fuculokinase | 3.195 |
| APL_1689 | fucO | Probable alcohol dehydrogenase, class IV | 2.887 |
| APL_1684 | fucI | l-Fucose isomerase | 2.812 |
| APL_1333 | hybB | Putative Ni/Fe hydrogenase 2 b-type cytochrome component | 2.796 |
| APL_1019 | kdgK | 2-Dehydro-3-deoxygluconokinase | 2.334 |
| APL_0896 | fdhE | Formate dehydrogenase accessory protein FdhE | 2.007 |
| Transport and binding proteins: cations and iron | |||
| APL_1793 | fecE | Fe(III) dicitrate ABC transporter, ATP-binding protein | 6.113 |
| APL_1955 | Outer membrane receptor proteins, mostly Fe transport | 3.271 | |
| APL_1981 | corA | Magnesium transport protein CorA | 3.001 |
| APL_0077 | exbD2 | Energy transducing protein ExbD2 | 2.346 |
| Transport and binding proteins: others | |||
| APL_0066 | dppC | Dipeptide transport system, permease components | 12.073 |
| APL_0870 | Putative C4-dicarboxylate transporter | 6.308 | |
| APL_1713 | Putative oligopeptide transporter | 4.639 | |
| APL_0191 | Predicted Na+-dependent transporters of the SNF family | 4.638 | |
| APL_0309 | yheS | Putative ABC transporter ATP-binding protein YheS | 2.331 |
| APL_1848 | cysA | Sulfate/thiosulfate import ATP-binding protein CysA | 1.938 |
| APL_1249 | sapF | Peptide transport system ATP-binding protein SapF | 1.858 |
| APL_0260 | modC | Molybdenum import ATP-binding protein ModC | 1.799 |
| APL_0582 | sotB | Putative efflux transporter | 1.773 |
| Regulatory functions | |||
| APL_1099 | Organic radical activating enzymes | 7.175 | |
| APL_1962 | hflX | GTP-binding protein HflX | 2.005 |
| Transcription | |||
| APL_0176 | pcnB | Putative poly(A) polymerase | 2.566 |
| Purines, pyrimidines, nucleosides, and nucleotides | |||
| APL_0775 | prsA | Ribose-phosphate pyrophosphokinase | 8.463 |
| APL_0425 | purF | Amidophosphoribosyltransferase | 1.812 |
| Protein fate | |||
| APL_1742 | srp54 | Signal recognition particle protein (sigma 54 like) | 6.861 |
| APL_1905 | dnaJ | Chaperone protein DnaJ | 3.383 |
| APL_1330 | hypF | Carbamoyltransferase HypF | 2.080 |
| Protein synthesis | |||
| APL_0034 | engD | GTP-dependent nucleic acid-binding protein EngD | 17.924 |
| APL_0575 | deaD | Superfamily II DNA and RNA helicases | 6.254 |
| APL_0487 | rplY | 50S ribosomal protein L25 (general stress protein Ctc) | 3.429 |
| APL_1325 | Putative 2-methylthioadenine synthetase | 2.357 | |
| APL_1112 | rumA | 23S rRNA (uracil-5-)-methyltransferase RumB | 2.145 |
| APL_0853 | Methionine synthase II (cobalamin independent) | 2.078 | |
| Cellular processes | |||
| APL_1922 | pgaB | Biofilm PGA synthesis lipoprotein PgaB precursor | 7.257 |
| APL_0011 | ftsL | Cell division protein FtsL | 3.349 |
| APL_1923 | pgaC | Biofilm PGA synthesis N-glycosyltransferase PgaC | 2.454 |
| Cell envelope | |||
| APL_0551 | tadB | Tight adherence protein B | 2.453 |
| APL_1841 | murI | Glutamate racemase | 2.340 |
| APL_0016 | murD | UDP-N-acetylmuramoy-l-alanine-d-glutamate synthetase | 2.113 |
| APL_1598 | mrdB | Rod-shape determining protein | 2.096 |
| APL_0419 | lgtF | UDP-glucose-lipooligosaccharide glucosyltransferase | 1.929 |
| APL_1554 | wecA | Putative undecaprenyl-phosphate α-N-acetylglucosaminyl 1-phosphate transferase | 1.577 |
| APL_0555 | rcpA | Rough colony protein A | 1.559 |
| Fatty acids and phospholipids metabolism | |||
| APL_1864 | accB | Biotin carboxyl carrier protein of acetyl-CoA carboxylaseb | 8.665 |
| APL_1865 | accC | Biotin carboxylase | 4.016 |
| APL_0887 | fadI | 3-ketoacyl-CoA thiolase | 3.675 |
| Amino acids biosynthesis | |||
| APL_0320 | metC | Cystathionine beta-lyase | 9.278 |
| APL_0099 | ilvG | Acetolactate synthase isozyme II large subunit | 6.897 |
| APL_1452 | serA | d-3-Phosphoglycerate dehydrogenase | 3.896 |
| APL_0469 | trpB | Tryptophan synthase beta chain | 3.274 |
| APL_0139 | leuC | 3-Isopropylmalate dehydratase large subunit 2 | 3.253 |
| APL_1951 | proA | Gamma-glutamyl phosphate reductase | 2.846 |
| APL_1147 | trpG | Putative anthranilate synthase component II | 2.723 |
| APL_0249 | thrB | Homoserine kinase | 2.459 |
| APL_1125 | Putative cysteine desulfurase | 2.068 | |
| DNA metabolism | |||
| APL_1196 | Type I site-specific restriction-modification system, R subunit | 3.251 | |
| APL_1194 | Type I restriction-modification system, methyltransferase subunit | 2.816 | |
| APL_1146 | rmuC | DNA recombination protein RmuC homolog | 2.462 |
| APL_0874 | holA | DNA polymerase III, delta subunit | 2.022 |
| APL_0287 | hsdM | Putative type I restriction-modification system, methyltransferase subunit | 2.005 |
| Central intermediary metabolism | |||
| APL_2045 | sseA | Probable thiosulfate sulfurtransferase | 1.942 |
| APL_1843 | cysJ | Sulfite reductase [NADPH] flavoprotein alpha component | 1.780 |
a
Seventy-nine genes.
b
CoA, coenzyme A.