TABLE 5.

A. pleuropneumoniae genes which are downregulated during adherence to SJPL cells^a

Locus tag	Gene	Description	Fold change
Hypothetical/unclassified/unknown
APL_1887		Esterase domain-containing protein	−6.273
APL_1284		Putative DNA-binding protein	−3.076
APL_0049		Hypothetical protein	−2.982
APL_0970		Hypothetical protein	−2.980
APL_1100		Hypothetical protein	−2.928
APL_1437		Hypothetical protein	−2.751
APL_0116		Hypothetical protein	−2.566
APL_0389	ompP4	Lipoprotein E precursor, predicted secreted acid phosphatase	−2.509
APL_0704		Potential type III restriction enzyme	−2.293
APL_1365	Hly	Hypothetical protein	−2.174
APL_0110		Hypothetical protein	−2.144
APL_1396		Hypothetical protein	−2.125
APL_0756		Hypothetical protein	−2.098
APL_0889		Hypothetical protein	−1.757
Energy metabolism
APL_0434	gapA	Glyceraldehyde-3-phosphate dehydrogenase	−4.981
APL_0892	fdxG	Formate dehydrogenase, nitrate-inducible, major subunit	−4.853
APL_0771	lpdA	Dihydrolipoyl dehydrogenase	−4.765
APL_1379	ccp	Cytochrome c peroxidase	−4.447
APL_0983	tktA	Transketolase 2	−3.790
APL_0894	fdxH	Formate dehydrogenase, iron-sulfur subunit	−3.627
APL_1251	pgk	3-Phosphoglycerate kinase	−3.625
APL_1450	fbp	Fructose-1,6-bisphosphatase	−3.315
APL_0181	gloA	Lactoylglutathione lyase	−3.046
APL_1925	tpiA	Triosephosphate isomerase	−3.023
APL_1091	aspA	Aspartate ammonia-lyase	−2.722
APL_0688	torZ	Trimethylamine-N-oxide reductase precursor	−2.687
APL_1011	adh2	Aldehyde-alcohol dehydrogenase 2	−2.642
APL_0772	aceF	Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex (E2)	−2.620
APL_1137	pgi	Glucose-6-phosphate isomerase	−2.595
APL_0486	maeA	NADP-dependent malic enzyme	−2.416
APL_1526	frdD	Fumarate reductase subunit D	−2.267
APL_0101	nrfB	Nitrate reductase, cytochrome-c-type protein	−2.231
APL_1250	fba	Fructose bisphosphate aldolase	−2.124
Transport and binding proteins: others
APL_1620	cbiO	Predicted ABC-type cobalt transport, ATPase component	−2.108
APL_0447	lctP	Putative l-lactate permease	−2.068
APL_0719		Putative phosphate permeases	−1.816
APL_0791		Transmembrane transport protein-permease	−1.702
Regulatory functions
APL_0656	hlyX	Regulatory protein HlyX	−2.727
APL_0629	cpxR	Transcriptional regulatory protein CpxR	−2.367
Purines, pyrimidines, nucleosides, and nucleotides
APL_0769	ushA	UshA precursor	−3.470
APL_0646	cpdB	2′,3′-Cyclic-nucleotide 2′-phosphodiesterase precursor	−2.778
APL_1014	deoD	Purine-nucleoside phosphorylase	−2.247
Protein fate
APL_1154		Putative zinc protease	−2.401
APL_1456	slyD	FkbP-type peptidyl-prolyl cis-trans isomerase	−2.313
Protein synthesis
APL_0740	rpsA	30s ribosomal protein S1	−1.608
Cellular processes
APL_1445	apxIC	RTX-I toxin-activating lysine-acyltransferase ApxIC	−4.117
APL_0956	apxIIA	RTX-II toxin determinant A	−4.052
APL_0004	sodC	Superoxide dismutase (Cu/Zn) precursor	−3.193
Cell envelope
APL_1494	ftpA	COG0783: DNA-binding ferritin-like protein (oxidative damage protectant)	−4.223
APL_0652	manB	Phosphomannomutase	−3.375
Central intermediary metabolism
APL_0645	ackA	Acetate kinase	−3.190
APL_1899	ppa	Inorganic pyrophosphatase	−2.207

^aFifty-two genes.