FIG. 2.

NF-κB and MAPK activation in wild-type and RICK-deficient macrophages infected with P. aeruginosa. Macrophages from wild-type (WT) and RICK-deficient (RICK knockout [KO]) mice were left untreated (−) or stimulated with LPS (100 ng/ml) for 24 h and then infected with P. aeruginosa at a macrophage/bacterium ratio of 1/10 for the indicated times (in minutes). (A) Whole-cell extracts were immunoblotted with antibodies that detect unphosphorylated and phosphorylated (activated) forms of the indicated proteins (p p38, phosphorylated p38). (B) The band density was determined by dividing the density of phosphorylated forms by unphosphorylated forms. The results of this experiment are representative of those of three independent experiments.