FIG. 2.
NT-3 specifically inhibits T. cruzi-TrkC binding. (A) A total of 2× molar amounts of recombinant NT-3, BDNF, or NGF (0.6 μg) to TrkCFc were incubated with 5 × 106 T. cruzi parasites and TrkCFc (1 μg). Parasites were then spun down and washed, and bound TrkC was revealed by Western blotting using anti-human IgG antibodies. (B) Dose response of increasing concentrations of NT-3 incubated with live T. cruzi parasites and TrkCFc. Bound TrkCFc was calculated by scanning densitometry, with each sample standardized against its parasite wash control. (Inset) A representative blot from the experiment is shown. Experiments in A and B were done three times, with similar results.