FIG. 3.

PDNF binds TrkC. (A) Receptor (1 μg) was incubated with PDNF (1 μg) and mixed with protein G-Sepharose to pull down Fc-receptors. Coimmunoprecipitated PDNF was revealed by Western blotting using TCN-2 monoclonal antibody. Total receptor was revealed using anti-human IgG antibodies. The additional blot shows 1 μg PDNF visualized by a Western blot developed using TCN-2 monoclonal antibody. (B) Parasites were incubated with 1 μg of TrkC^Fc and increasing concentrations of PDNF. Parasites were washed and evaluated for bound TrkC^Fc. Bound TrkC^Fc was calculated by densitometry, with each sample standardized against its parasite wash control, as revealed with chagasic serum (one of several bands shown). Experiments in A and B were done three times, with similar results.