FIG. 6.

PDNF promotes TrkC-dependent neuronal and glial cell survival. (A) NNR5^TrkC, NNR5^TrkB, and NNR5^EV cells were plated in triplicate and cultured for 3 days in 1% FCS, serum-free DMEM, or serum free-medium supplemented with PDNF (250 ng/ml). Cells were stained with propidium iodide and Hoechst nuclear staining reagents and counted by fluorescence microscopy (magnification, ×20). (B) Graph of data for experiment shown in A with PDNF survival normalized against serum-free survival. Error bars represent the standard errors of the means between experiments; an average of three experiments were performed, with each point in triplicate. (C) Schwann cells were plated in triplicate and then cultured under conditions as indicated: 1% FBS or serum free with or without anti-TrkC (1 μg/ml), anti-TrkB (1 μg/ml), PDNF (250 ng/ml), or a combination. Error bars represent the variation between experiments. P values (analysis of variance) were calculated using Prism software. An average of two experiments were performed, each point in triplicate.