FIG. 5.

Increased numbers of inflammatory foci in the basal ganglia of intranasally infected compared to subcutaneously infected mice. C57BL/6 mice were infected intranasally (i.n.) with 25 × 10³ or subcutaneously (s.c.) with 5 × 10³ T. cruzi parasites, and at 25 days PI, their brains were fixed in formalin, embedded in paraffin, sectioned both coronally and sagittally, and stained with H&E. Three noncontinuous brain sections (basal ganglia) were counted per mouse. From each section, 25 fields within the basal ganglia (magnification, ×200) were counted. The average number of parasite nests per 25 fields for each mouse was determined. The numbers of T. cruzi nests (foci) were calculated from the averages for three mice. **, P < 0.001.