TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| Top 10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 ara139 Δ(ara-leu)7697 galU galK rpsL(Strr) endA1 nupG | Invitrogen |
| Origami B (DE3) | F−ompT hsdSB (rB− mB−) gal dcm lacY1 ahpC (DE3) gor522::Tn10trxB (Kanr Tetr) | Novagen |
| BL21(DE3) | F−ompT hsdSB (rB− mB−) gal dcm (DE3) | Novagen |
| Plasmids | ||
| pMCS69 | pBBR1MCS derivative containing phaA and phaB genes from C. necator | 2 |
| pET14b | Apr; T7 promoter | Novagen |
| pETC | Plasmid coding for PhaC wild type under the control of T7 promoter | 20 |
| pET14b-SAphaC | Plasmid coding for streptavidin(SA)-PhaC fusion under the control of T7 promoter | 20 |
| pET 14b-BLAphaC | Plasmid coding for BLA(+ss)PhaC fusion under the control of T7 promoter | This study |
| pET14b-BLA(−ss)phaC | Plasmid coding for BLA(−ss)PhaC fusion under the control of T7 promoter | This study |