TABLE 2.
Methanogen 16S rRNA gene libraries constructed prior to vaccination and 4 weeks after the second vaccination
| 16S rRNA clone namea | GenBank accession no. | Nearest valid related organism | % Sequence identity | Related vaccine strain | % Sequence identity | Predicted % cross reactivityb | No. of clones per library
|
||
|---|---|---|---|---|---|---|---|---|---|
| Before expt | Postvaccination
|
||||||||
| Control | Treatment | ||||||||
| CSIRO-WA101 | AY351466 | Thermoplasma volcanium | 78.6 | ZA-10 | 76.1 | <0.1 | 31 | 2 | |
| CSIRO-WA04 | AY615202 | Methanobrevibacter ruminantium M1 | 99.0 | AK87 | 100.0 | 100.0 | 24 | ||
| CSIRO-WA03 | AY196669 | Methanobrevibacter smithii PS | 100.0 | 1Y | 98.2 | 89.9 | 18 | 5 | 3 |
| CSIRO-WA012 | AJ009958 | Methanobrevibacter millerae ZA-10 | 99.1 | ZA-10 | 99.1 | 94.1 | 18 | 29 | 22 |
| CSIRO-WA02 | AB034185 | Methanobrevibacter millerae ZA-10 | 98.4 | 1Y | 99.3 | 95.0 | 12 | 9 | 4 |
| CSIRO-WA083 | AY995282 | Thermoplasma acidophilum | 74.2 | AK87 | 76.7 | <0.1 | 9 | ||
| CSIRO-WA07 | AY196679 | Methanomicrobium mobile BP | 100.0 | BP | 100.0 | 100.0 | 8 | ||
| CSIRO-WA064 | DQ123883 | Methanosphaera stadtmanae MCB-3 | 95.8 | MCB-3 | 95.8 | 78.9 | 7 | ||
| CSIRO-WA125 | AY995292 | Thermoplasma acidophilum | 74.1 | AK87 | 75.9 | <0.1 | 2 | 1 | |
| CSIRO-WA056 | DQ123877 | Methanimicrococcus blatticola | 89.8 | BP | 79.6 | <5.0 | 1 | ||
| CSIRO-WA097 | AY995280 | Thermoplasma acidophilum | 72.7 | AK87 | 75.3 | <0.1 | 1 | ||
| CSIRO-WA118 | AY995300 | Thermoplasma volcanium | 73.5 | 1Y | 75.2 | <0.1 | 1 | ||
| CSIRO-WA13 | EU093103 | Methanobrevibacter millerae ZA-10 | 98.8 | ZA-10 | 98.8 | 92.7 | 1 | ||
| CSIRO-WA14 | EU093104 | Methanobrevibacter millerae ZA-10 | 99.0 | ZA-10 | 99.0 | 93.6 | 1 | ||
| CSIRO-WA15 | EU093105 | Methanobrevibacter millerae ZA-10 | 98.9 | ZA-10 | 98.9 | 93.2 | 1 | ||
| CSIRO-WA199 | AY351494 | Methanobrevibacter millerae ZA-10 | 99.0 | ZA-10 | 99.0 | 93.6 | 1 | ||
| CSIRO-WA20 | EU093107 | Methanobrevibacter millerae ZA-10 | 98.9 | ZA-10 | 98.9 | 93.2 | 1 | ||
| CSIRO-WA1610 | AY351434 | Methanobrevibacter millerae ZA-10 | 96.3 | AK87 | 97.0 | 84.4 | 2 | 18 | |
| CSIRO-WA1711 | AY351493 | Methanobrevibacter millerae ZA-10 | 99.8 | ZA-10 | 99.8 | 97.3 | 1 | ||
| CSIRO-WA18 | EU093106 | Methanobrevibacter smithii PS | 97.8 | 1Y | 98.7 | 92.2 | 7 | ||
| CSIRO-WA21 | EU093108 | Methanobrevibacter wolinii SH | 95.4 | AK87 | 93.5 | 68.4 | 2 | ||
| CSIRO-WA22 | EU093109 | Methanobrevibacter millerae ZA-10 | 83.6 | ZA-10 | 83.6 | 22.9 | 1 | ||
| CSIRO-WA23 | EU093110 | Methanobrevibacter wolinii SH | 95.3 | AK87 | 93.3 | 67.4 | 1 | ||
| Totalc | 132 | 57 | 55 | ||||||
a
Alternative designations are represented by superscript numerals as follows: 1, CSIRO1.33; 2, SM9; 3, CSIRO-Qld09; 4, ON-CAN.13; 5, CSIRO-Qld24; 6, ON-CAN.05; 7, CSIRO-Qld07; 8, CSIRO-Qld34; 9, CSIRO3.06; 10, CSIRO3.07; and 11, CSIRO3.05.
b
Based upon the linear regression equation Y = 360.81284 − 4.59014X (where X equals the percent sequence identity).
c
Total number of clones examined in each library.