TABLE 2.
Results of sequencing of representative 16S rRNA gene clones
| Clone | Accession no. | Highest BLAST hit (accession no.)/% identity | Taxonomic affiliation according to RDP |
|---|---|---|---|
| B3 (471) | EU850359 | Uncultured betaproteobacterium clone S7 (AF447793)/98 | Rhodocyclaceae |
| C10 (475) | EU850366 | Uncultured betaproteobacterium clone nsc151 (DQ211501)/100 | Rhodocyclaceae |
| C11 (501) | EU850367 | Uncultured bacterium SA34 (AF245349)/99 | Rhodocyclaceae |
| D7 (525) | EU850371 | Uncultured bacterium clone UTFS-002-12-23 (AB166771)/99 | Rhodocyclaceae |
| D10 (483) | EU850372 | Uncultured bacterium clone UTFS-002-12-23 (AB166771)/99 | Rhodocyclaceae |
| E3 (468) | EU850376 | Uncultured bacterium SA34 (AF245349)/99 | Rhodocyclaceae |
| E4 (477) | EU850377 | Uncultured bacterium clone VIR_D5 (EF565151)/99 | Rhodocyclaceae |
| E6 (458) | EU850379 | Uncultured bacterium clone VIR_D5 (EF565151)/99 | Rhodocyclaceae |
| E8 (516) | EU850381 | Uncultured bacterium SA34 (AF245349)/98 | Rhodocyclaceae |
| F5 (497) | EU850384 | Uncultured bacterium clone UTFS-002-12-23 (AB166771)/99 | Rhodocyclaceae |
| F11 (485) | EU850389 | Uncultured bacterium clone VIR_D5 (EF565151)/98 | Rhodocyclaceae |
| H5 (512) | EU850394 | Uncultured bacterium clone D07 (EF589969)/97 | Rhodocyclaceae |
| OTUa 1 | 27 clones | Rhodocyclaceae | |
| A1 (425) | EU850352 | Pseudomonas putida KT2440 (AE015451)/99 | Pseudomonas spp. |
| E5 (443) | EU850378 | Pseudomonas putida isolate 24 (EU438854)/99 | Pseudomonas spp. |
| E12 (515) | EU850383 | Pseudomonas putida BM2 (DQ989291)/99 | Pseudomonas spp. |
| OTU 2 | 13 clones | Pseudomonas spp. | |
| B1 (291) | EU850358 | Uncultured Nitrospira sp. clone 0B11 (EU499597)/98 | Nitrospira spp. |
| B8 (578) | EU850362 | Uncultured Nitrospira sp. clone 3 (DQ414437)/99 | Nitrospira spp. |
| E2 (463) | EU850375 | Uncultured Nitrospira sp. clone 3 (DQ414437)/99 | Nitrospira spp. |
| OTU 3 | 7 clones | Nitrospira spp. | |
| A2 (407) | EU850353 | Uncultured gammaproteobacterium clone GB2917y (DQ201885)/96 | Gammaproteobacteria |
| A5 (389) | EU850355 | Uncultured gammaproteobacterium clone GB2917y (DQ201885)/95 | Gammaproteobacteria |
| A7 (477) | EU850357 | Uncultured gammaproteobacterium clone GB2917y (DQ201885)/94 | Gammaproteobacteria |
| OTU 4 | 7 clones | Gammaproteobacteria | |
| A3 (485) | EU850354 | Uncultured eubacterium clone F13.46 (AF495440)/98 | Tetrasphaera spp. |
| E9 (451) | EU850382 | Uncultured bacterium clone Ebpr19 (AF255629)/99 | Tetrasphaera spp. |
| F9 (473) | EU850387 | Uncultured eubacterium clone F13.46 (AF495440)/98 | Tetrasphaera spp. |
| OTU 5 | 4 clones | Tetrasphaera spp. | |
| A6 (447) | EU850356 | Dechloromonas sp. strain A34 (EF632559)/97 | Dechloromonas spp. |
| D11 (504bp) | EU850373 | Uncultured bacterium clone ORS10C_g11 (EF392932)/99 | Dechloromonas spp. |
| E7 (535) | EU850380 | Dechloromonas sp. strain A34 (EF632559)/97 | Dechloromonas spp. |
| OTU 6 | 4 clones | Dechloromonas spp. | |
| B7 (448) | EU850361 | Uncultured actinobacterium clone DOK_NOFERT_clone341 (DQ829293)/96 | Actinomycetales |
| F8 (450) | EU850386 | Uncultured actinobacterium clone DOK_NOFERT_clone341 (DQ829293)/96 | Actinomycetales |
| OTU 7 | 2 clones | Actinomycetales | |
| E1 (438) | EU850374 | Uncultured bacterium clone T015D (AM158382)/91 | Candidate division TM7 |
| H2 (428) | EU850393 | Uncultured bacterium clone IC-61 (AB255073)/93 | Candidate division TM7 |
| OTU 8 | 2 clones | Candidate division TM7 | |
| C12 (530) | EU850368 | Uncultured bacterium clone DSSD59 (AY328757)/98 | Alphaproteobacteria |
| G12 (559) | EU850392 | Derxia gummosa (AB089482)/95 | Betaproteobacteria |
| C2 (413) | EU850365 | Uncultured bacterium clone 197up (AY212650)/99 | Burkholderiales |
| B10 (532) | EU850363 | Ralstonia detusculanense (AF280433)/99 | Ralstonia spp. |
| D1 (452) | EU850369 | Uncultured bacterium clone TH-141 (AB184983)/98 | Comamonadaceae |
| F7 (457) | EU850385 | Uncultured bacterium clone 44 (DQ413103)/99 | Rhodocyclaceae |
| G10 (449) | EU850391 | Uncultured bacterium clone SRRB48 (AB240518)/96 | Nocardioidaceae |
| B5 (458) | EU850360 | Uncultured Sphingobacteria bacterium clone ADK-SGe02-50 (EF520599)/96 | Sphingobacteriales |
| B11 (522) | EU850364 | Uncultured bacterium clone HM15 (AM909923)/95 | Acidobacteriaceae |
| F10 (495) | EU850388 | Uncultured bacterium clone LaP15L89 (EF667686)/99 | Caldilinea spp. |
| D5 (435) | EU850370 | Uncultured bacterium clone mdt16a02 (AY537009)/98 | Bacteria |
| F12 (574) | EU850390 | Uncultured bacterium clone 032D06_P_BA_P3 (BX294877)/92 | Bacteria |
a
OTU, operational taxonomic unit.