FIG. 6.
Transfer of RBS toxicity in P. furiosus membrane fractions. Membranes from cultures treated with 20 μM RBS were added to fresh P. furiosus cultures with increasing injection volumes (μl). Each culture was monitored by cell count after a 30-min incubation with the RBS/membrane preparation. Symbols are as follows: ▪, no membranes added; •, RBS-treated membranes added; ⧫, RBS-treated membranes added after prior incubation with S0 (∼4 mM) at 98°C for 30 min; ▴, RBS-treated membranes added after prior incubation with polysulfide (4 mM) at 98°C for 30 min. Cultures with cell densities below 1 × 106 cells/ml were considered not viable.