TABLE 1.
Strains, plasmids, and cloning primers used in this study
| Strain, plasmid or primer | Genotype, description, or sequence | Source or reference |
|---|---|---|
| Strains | ||
| S. cerevisiae | ||
| PSY580a (wild type) | Mataura3Δ52 trp1Δ63 leu2Δ1GAL2+ | 47 |
| PSY3643 | PSY580a Δfdh1::loxP-kanMX-loxP | This work |
| PSY3644 | PSY580a Δfdh1::loxP | This work |
| PSY3645 | PSY580a Δfdh1::loxP Δfdh2::loxP-kanMX-loxP | This work |
| PSY3646 (ΔFDH1 ΔFDH2) | PSY580a Δfdh1::loxP Δfdh2::loxP | This work |
| PSY3647 | PSY3646 LEU2::pflB | This work |
| PSY3648 (PFL) | PSY3646 LEU2::pflBTRP1::pflA | This work |
| PSY3649 (PFL and AdhE) | PSY3646 LEU2::pflBTRP1::pflAURA3::AdhE | This work |
| E. coli | ||
| W3110 | F− λ− IN(rrnD-rrnE)1 rph-1 | ATCC 27325 |
| Plasmids | ||
| pUG6 | Deletion plasmid; kanMX marker | 15 |
| pSH65 | Cre-expressing plasmid; phleomycin gene marker | 15 |
| V0120-BB | Empty biobrick vector | 29 |
| P0511-BB | pGal1 | 2 |
| L0101-BB | Adh1 terminator | 2 |
| PPS3160-BB | V0120; pflB | This work |
| PPS3161-BB | V0120; pflA | This work |
| PPS3162-BB | V0120; adhE | This work |
| PPS3163-BB | V0120; pGal1-pflB-Adh1_Term | This work |
| PPS3164-BB | V0120; pGal1-pflA-Adh1_Term | This work |
| PPS3165-BB | V0120; pGal1-adhE-Adh1_Term | This work |
| pRS304 | S. cerevisiae integration vector; trp1 marker | 38 |
| pRS305 | S. cerevisiae integration vector; leu2 marker | 38 |
| PRS306 | S. cerevisiae integration vector; ura3 marker | 38 |
| PPS3166 | pRS304; pGal1-pflA-Adh1_Term | This work |
| PPS3167 | pRS305; pGal1-pflB-Adh1_Term | This work |
| PPS3168 | pRS306; pGal1-adhE-Adh1_Term | This work |
| Cloning primers | ||
| FDH1/2-hom1 | ATGTCGAAGGGAAAGGTTTTGCTGGTTCTTTACGAAGGTGCAGCTGAAGCTTCGTACGC | This work |
| FDH1/2-hom2 | TTATTTCTTCTGTCCATAAGCTCTGGTGGCATAAGAACCAGCATAGGCCACTAGTGGATCTG | This work |
| 5′ pflB | CCTTGAATTCGCGGCCGCATCTAGAATGTCCGAGCTTAATGAAAAG | This work |
| 3′ pflB | AAGGACTAGTTTACATAGATTGAGTGAAGGTACGAGTAATAACGTCCTGCTG | This work |
| 5′ pflA | CCTTGAATTCGCGGCCGCATCTAGAATGTCAGTTATTGGTCGCAT | This work |
| 3′ pflA | AAGGACTAGTTTAGAACATTACCTTATGACCGTACTGCTCAAGAATGCC | This work |
| 5′ adhE | CCTTGAATTCGCGGCCGCATCTAGAATGGCTGTTACTAATGTCG | This work |
| 3′ adhE | AAGGACTAGTTTAAGCGGATTTTTTCGCTTTTTTCTCAGCTTTAGCCGG | This work |