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Purification of plasma membranes by two-phase partitioning
| Enzymatic marker(s) | Sp act (nmol min−1 mg of protein−1) ± SD in:
|
% in plasma membranes ± SD | |
|---|---|---|---|
| Total cell membranes | Plasma membranes | ||
| Cytochrome c reductase (endoplasmic reticulum) | 340 ± 21 | 24.2 ± 3.9 | 0.9 ± 0.6 |
| Cytochrome c oxidase (mitochondria) | 137 ± 23 | 19.2 ± 8.6 | 4.5 ± 2.7 |
| Glucan synthase (plasma membrane) | 1.9 ± 0.47 | 14.4 ± 2.5 | 70.5 ± 6.7 |
| Proteins | 11.8 ± 3.2 | ||
