FIG. 1.

Negative image composition of SybrGold-stained DGGE gels of environmental ITS1 genes of fungi obtained with the specific primer set ITS-1F-ITS2. The dendrogram on top is based on a similarity matrix (Dice index) calculated from a presence-absence binary matrix of bands. Grouping was based on the UPGMA method. Bands indicated with an arrow head were excised and sequenced. REF, reference position markers for gel comparison; D_ME-TCA, TCA tainted cork discs; D_C, control untainted cork discs.