FIG. 2.

Negative image composition of SybrGold-stained DGGE gels of environmental ITS1 genes of fungi obtained with the specific primer set ITS-1F-ITS2 from cork stoppers. The dendrogram on top is based on a calculated similarity matrix (Dice index) from a presence-absence binary matrix of bands. Grouping has been made by using a UPGMA method. Bands indicated with an arrow head were excised and sequenced. REF, reference position markers for gel comparison; ME, ME tainted cork stoppers; C, control untainted cork stoppers.