TABLE 3.
Most probable sequence identification and occurrence of DGGE bands in control and ME tainted cork samples: closest bacterial speciesa
| Group and band no.b | Closest bacterial species | % Presence in cork samples
|
|
|---|---|---|---|
| C (n = 13) | ME (n = 13) | ||
| Group 1 | Alphaproteobacteria | ||
| EU105, EU106 | Methylobacterium B25 (EU240206) | 38.5 | 22.1 |
| EU83, EU129 | Novosphingobium subarcticum LH128 (AY151394) | 46.2 | |
| EU125, EU86 | Paracoccus marcusii (Y12703) | 15.4 | 30.8 |
| EU126 | Paracoccus sp. strain B61Y (EU368762) | 15.4 | |
| EU117 | Sphingobium yanoikuyae BF-18 (EU307932) | 7.7 | |
| EU77, EU98, EU99, EU102, EU114, EU128, EU137 | Sphingobium yanoikuyae DD109 (AY574367) | 100.0 | 100.0 |
| EU87 | Sphingobium sp. strain 2F5-2 (EU286535) | 30.8 | |
| EU115 | Sphingomonas sp. strain D31C2 (AY162145) | 30.8 | 15.4 |
| Group 2 | Betaproteobacteria | ||
| EU132 | Zooglea sp. strain AI-20 (AY437629) | 7.7 | |
| Group 3 | Gammaproteobacteria | ||
| EU12, EU34, EU75, EU76, EU124 | Acinetobacter sp. strain SH825131.2 (AJ633636) | 53.8 | 100.0 |
| EU24 | Acinetobacter lwofii PTA-152 (AM293676) | 15.4 | |
| EU78, EU80, EU138 | Pseudomonas putida NABI (AY395005) | 46.2 | |
| EU122 | Pseudomonas aeruginosa SG-1 (EU391389) | 15.4 | |
| EC01, EC02, EC03, EC04, EC05, EC06 | Pseudomonas sp. strain pl-72 (EU375376) | 46.2 | |
| Group 4 | Actinobacteria | ||
| EU108 | Brachybacterium paraconglomeratum GN0406 (DQ890508) | 7.7 | |
| EU97, EU134 | Microbacterium oxydans H190 (EF204430) | 30.8 | |
| EU135 | Microbacterium sp. strain Atl-19 (EF028128) | 7.7 | |
| Group 5 | Bacteroidetes | ||
| EU08, EU133 | Flavobacterium sp. strain THWCSN41 (AM888190) | 7.7 | 46.2 |
a
Control (C) and ME tainted cork samples were examined. The closest matches (>98% similarity) to sequences of cultured bacteria in GenBank database are indicated. Accession numbers appear in parentheses in column 2. n, number of samples.
b
Bands are grouped based on the division or phylum categorizations in column 2.