FIG. 5.

RT-PCR results for wild-type strain M145, the rrdA null mutant, the rrdA high-expression strain, and redD null mutant K68. (A) Transcriptional levels of the pathway-specific activators, including redD (primers Oxj201 and Oxj202, 368 bp), redZ (primers Oxj203 and Oxj204, 322 bp), actII-orf4 (primers Oxj205 and Oxj206, 320 bp), rrdA [primers Oxj211 and Oxj212x, 166 bp; the primer sequences are located in the upstream region of the insertion site of the aac(3)-IV fragment used for gene disruption], and hrdB (control) (primers Oxj237 and Oxj238, 207 bp). (B) RT-PCR results for the redD null mutant K68 and wild-type strain M145 for transcriptional detection of actII-orf4. WT, wild type.