TABLE 2.

Primers used in genetic modifications

Primer name	Sequencea
Pr-AflIII.fwd	5′-GCATAACCAGCATAAACGTGTCCGGTTTGATAG-3′
Pr-AflIII.rev	5′- CGATAACCAGCATAAACGTGTCCGGTTTGATAG-3′
Pr-BspLU11I.fwd	5′-CTTAGGCTTTATGCAACATGTACAATAATAATGAAGTCATG-3′
Pr-BspLU11I.rev	5′-CATGACTTCATTATTATTGTACATGTTGCATAAAGCCTAAG-3′
Pr-Pm1.seq	5′-GCATAACCAGCATAAACGTGTCCGGTTTGATAG-3′
Pr-Pm1.fwd	5′-CTTTCACCAGCGTTTCTGGGTG-3′
Pr-Pm1.rev	5′-GATGTAGAAAGGCGCCAAGTC-3′
Pr-Pm2.fwd	5′-CGACACGGAAATGTTGAATACTCATATGTT-3′
Pr-Pm2.rev	5′-GCATAACCAGCATAAACGTGTCCGGTTTGATAG-3′
Pr-Pm2.seq	5′-CTTTCACCAGCGTTTCTGGGTG-3′
Pr-bla-rt.fwd	5′-TGGATCTCAACAGCGGTAAG-3′
Pr-bla-rt.rev	5′-GGCAGCACTGCATAATTCTCT-3′
Pr-luc-rt.fwd	5′- TGGAGAGCAACTGCATAAGG-3′
Pr-luc-rt.rev	5′-CGCAACTGCAACTCCGATAA-3′
Pr-celB-rt.fwd	5′- CGCTGACCACCAGCTTCAAT-3′
Pr-celB-rt.rev	5′-TGCTGCGGTCACGGTTATAG-3′
Pr-km-rt.fwd	5′-GCAATCAGGTGCGACAATCT-3′
Pr-km-rt.rev	5′-TCGCAGTGGTGAGTAACCAT-3′
Pr-ext-bla	5′-CCGCTGTTGAGATCCAGTTCG-3′
Pr-bla.fwd	5′-GCATAACCAGCATAAACGTGTCCGGTTTGATAG-3′
Pr-bla.rev	5′-GTGTATGCGGCGACCGAGTT-3′

^aIntroduced mutations are underlined, and restriction sites are shown in italics.