Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Production yields of soluble and insoluble recombinant GM-CSF using the Pm wild type and three selected Pm up-mutants
| Vector | Production yield of GM-CSF (g/liter)
|
||
|---|---|---|---|
| Soluble | Insoluble | Total | |
| pGM29pelB (wild-type Pm) | 0.32 ± 0.03 | 0.3 ± 0.06 | 0.6a |
| pMV1 (Pm ML1-18) | 0.43 ± 0.05 | 0.6 ± 0.12 | 1.0 |
| pMV2 (Pm ML1-16) | 0.47 ± 0.05 | 0.7 ± 0.14 | 1.2 |
| pMV3 (Pm ML1-14) | 0.50 ± 0.05 | 1.1 ± 0.22 | 1.6 |
The GM-CSF production level given here is in the same range as the analogous data presented previously (31).
