TABLE 2.

Primers used in this study

Name	Location/purpose	PCR primer sequence (5′-3′)
Reg.1 F	OEOE_0684, upstream of region 1	TACGAAGATGGTATTAAAGCGGTTA
Reg 1 R	OEOE_0691, downstream of region 1	TATCAAAATCAAGATCTTTTTCCAA
2.1 F	Region 2, checking of 3-oxo-acyl carrier protein reductase	CATCTTTTGAAAGGAGACAA
2.1 R	Region 2, checking of 3-oxo-acyl carrier protein reductase	TTAAGGCACCAAGATTTTTA
2.2 F	Region 2, checking of HP YP_809753	GGCTGCTGAGAAATCTATTA
2.2 R	Region 2, checking of HP YP_809753	TTTTAGAAAACGGTAAACCA
Reg 4 F	Region 4, left end, IS30	ACACCATTTTAAAGGAAGC
Reg 4 R	Region 4, right end, intergenic fnr/IS30	AGGAGCTGTTAGTGCCTGTA
4.1. F	Region 4, checking of HP NP_786185	GTCCTATGCCGACTTCCA
4.1. R	Region 4, checking of HP NP_786185	ACTGAAGACTGCGTTAGCTC
4.2. F	Region 4, checking of cadA ATPase	TCAAGATACCAAGTCCAAA
4.2. R	Region 4, checking of cadA ATPase	TAAGACTGCTAAAACGAGGA
4.3. F	Region 4, checking of dpsB	ACGGACATTCTGATCTAACA
4.3. R	Region 4, checking of dpsB	TTACGTTAAGTTGACCCAAG
4.4. F	Region 4, checking of copper chaperone	TTAACCTTGACGTTTTCAAC
4.4. R	Region 4, checking of copper chaperone	AGCAAGGTAAAGGCTAACTT
4.5. F	Region 4, checking of fnr	CTTAGTACCGACGCATGTTT
4.5. R	Region 4, checking of fnr	GTTTAAGACCTTGATGCTGA
Reg 5 F	Region 5, left end	AAACGCATTTTGTTTAATAGCATCT
Reg 5 R	Region 5, right end	CTACGCATGATTCCTCATTATCTCT
Reg 6 R	Region 6, right end (PTS IIA)	AGGGAGATGTTTCCAATGAAGA
OmrA F	RT-qPCRa	GTACCGGTTACAGCTCTA
OmrA R	RT-qPCR	GCCATAGCATCCTGAGT
Dldh F	RT-qPCR	TGAACAATGGGGAAAAGAGC
Dldh R	RT-qPCR	AATTGCATCATAGCCCTTGG

^aRT-qPCR, reverse transcription-quantitative PCR.