TABLE 5.
Frequency of EBV-infected cells in pooled scraped GCs compared to estimates in GCs isolated based on CD10 expression and flow cytometry
| Donor no. | Frequency of infected cells/105 GC cells based on:
|
|
|---|---|---|
| Flow cytometrya | Dissectionb | |
| 10 | 1.2 | 7.4 |
| 11 | 0.9 | 2.8 |
| 12 | 1.3 | 1.4 |
a
Estimated based on limiting dilution analysis of FACS-separated CD10+ cells from the same donor using DNA PCR for the W-repeat genomic sequence of EBV.
b
Estimated from the CD10 and EBER1 RT-PCR signals quantitated using serial dilutions of CD10+ cells from the same tonsil or IB4 cells (see text for details).