Neighbor-joining tree of env sequences from each of the 69 study participants from South Africa and Malawi. To limit the size of the tree, only 626 sequences of the 1,505 sequences generated were analyzed: where individuals harbored viruses with multiple identical env sequences, only the unique sequences were included. Red branches represent sequences from participants infected with more than one variant. Diamonds represent sequences with >6% divergence from the participant sequence population. The sequences for participants 703010131 and 703010159, a donor-recipient pair, cluster together, as indicated.