. 2009 Feb 11;83(8):3668–3683. doi: 10.1128/JVI.02063-08

TABLE 1.

Primers for mutagenesis

Mutation	Forward primer	Reverse primer
S32A	`CCATACCAAAGCCGGCGCCTTGGGCACCG`	`CGGTGCCCAAGGCGCCGGCTTTGGTATGG`
S43A S44A	`CGAAGAAACACAGACGACTAGCCGCCGACCAAGATCAGAGCCAG`	`CTGGCTCTGATCTTGGTCGGCGGCTAGTCGTCTGTGTTTCTTCG`
S49A	`CCAGCGACCAAGATCAGGCCCAGACACCGGAAACCCC`	`GGGGTTTCCGGTGTCTGGGCCTGATCTTGGTCGCTGG`
T51A	`CAAGATCAGAGCCAGGCACCGGAAACCCCTG`	`CAGGGGTTTCCGGTGCCTGGCTCTGATCTTG`
T54A	`CCAGACACCGGAAGCCCCTGCCACACC`	`GGTGTGGCAGGGGCTTCCGGTGTCTGG`
T57A	`CGGAAACCCCTGCCGCACCACTAAGTTGTTG`	`CAACAACTTAGTGGTGCGGCAGGGGTTTCCG`
T57D	`GACACCGGAAACCCCTGCCGACCCACTAAGTTGTTGCACAG`	`CTGTGCAACAACTTAGTGGGTCGGCAGGGGTTTCCGGTGTC`