FIG. 3.

NS2 inhibits TRIF-induced IFN promoter activity. 293T cells were transfected by an expression construct encoding TRIF and the pIFNβ-luc (a) or pISG56-luc (b) reporter constructs with increasing amounts of NS2 plasmids as in Fig. 2. Luciferase activities were determined at 24 h posttransfection. Shown are the means (± standard errors of the means) of triplicate samples. One representative experiment (of three) is shown. Asterisks denote a P value of <0.01 versus that of the control by one-way ANOVA. (c) The expression levels of TRIF, NS2, and β-actin were determined by WB analysis. (d) The ISG56 reporter assay was performed as described above using TRIF to induce promoter activity and increasing amounts of an expression plasmid encoding P. (e) WB analysis of protein expression from the experiment described for panel d.