FIG. 4.

DNA replication of PrV mutants. RK13 cells were infected with PrV-ΔUL6F, PrV-ΔUL15F, PrV-ΔUL28F, PrV-ΔUL32F, PrV-ΔUL33F, or wild-type PrV-Ka at an MOI of 5, and total DNA was prepared 24 h p.i. After BamHI cleavage Southern blots were hybridized with the labeled terminal BamHI fragment 13 of the PrV genome, which permits differentiation of packaged unit size DNA (B 13) and the concatemeric primary replication products (B 13+14′). A fragment (B 8′) from the internal inverted-repeat sequences was also detected by the probe (Fig. 1A). The sizes of marker DNAs are indicated.