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. 2009 Feb 4;83(8):3930–3943. doi: 10.1128/JVI.02636-08

FIG. 8.

FIG. 8.

trans-Complementation of UL32-negative PrV by the homologous HSV-1 protein. One-step growth kinetics were determined after infection of RK13 (A) or RK13-HUL32 (B) cells with PrV-Ka, PrV-ΔUL32F, pHSV-1ΔgJ, or pHSV-1ΔUL32KF at an MOI of 5. At the indicated times, progeny virus titers were determined by plaque assays on RK13-HUL32 cells. Shown are the mean results of three experiments. (C) Cell-to-cell spread was investigated 48 h after infection of RK13 or RK13-HUL32 cells at low MOI. The average diameters of 30 plaques each were calculated, and the plaque sizes of PrV-ΔUL32F and pHSV-1ΔUL32 were displayed as percentages of those of PrV-Ka and pHSV-1ΔgJ on the same cell line, which were set at 100%.