TABLE 1.
Primers used in this study
| Primer | Sequence (5′-3′)a | Origin and modification |
|---|---|---|
| PP82 | CAGTGTTGGCTTGCAAACTAG | PVX |
| PP228 | GGGTAAGTTTTCCGTATGTTG | GFP |
| PP267 | AGAAAAGTCATGAAGGTTCTGCTAGCCACGG | TCV |
| PP269 | CAAGGTGCGCGAGGTTTACCAATC | PVX |
| PP271 | CGGCTACCACATCCAAGGAAGG | 18S rRNA |
| PP272 | GAGCTGGAATTACCGCGGCTG | 18S rRNA |
| PP354 | TCAAGATCCGGAATGTCTATAAATATAAGAGACCC | FT, BspEI |
| PP355 | TCAAGATCCGGATAGTCTATAAATATAAGAGACCC | FT, BspEI |
| PP356 | AGGAAGAAGTCGACTAAAGTCTTCTTCCTCCGCAG | FT, SalI |
| PP371 | ATGAGTAAAGGAGAAGAACTTTTCACTGGAG | GFP |
| PP372 | TTTGTGTCCAAGAATGTTTCCATCTTC | GFP |
| PP373 | GTATGCTGTTTCCGTTGTGATCTCTGTGAG | PVX |
| PP407 | TTCAAGTGATCAGTAGTCTATAAATATAAGAGAC | FT, BclI |
| PP408 | AAGGAAGTTTAAACCTAAAGTCTTCTTCCTCCGCA | FT, PmeI |
| PP433 | ATGCCTCTCCTACATACACTCAACACAG | TCV |
| PP434 | GTCTACGACATGTCTCTCTTTGCAG | TCV |
| PP473a | TTGGCCATAAGTAACCTTTAG | FT |
| PP512 | TCAAGATGATCATAGTCTATAAATATAAGAGACCC | FT, BclI |
| PP513 | TTAGTCACGTTTAAACGGCCATAAGTAACCTTTAG | FT, PmeI |
| PP514 | GTTACTTGATCATAGAGAGAGGTGACTAATGGCTT | FT, BclI |
| PP515 | AGGAAGTTTAAACCTAAAGTCTTCTTCCTCCGCAG | FT, PmeI |
| PP516 | AGATCCGTTTAAACCAAAGTATAGAAGTTCCTGAG | FT, PmeI |
| PP517 | AACTTCTGATCATGAGTTATGGTGGATCCAGATG | FT, BclI |
| PP518 | ATCTCAGTTTAAACGGTTGTTCCAGTTGTAGCAGG | FT, PmeI |
| PP519 | ACAACTTGATCATAGTTTGGCAATGAGATTGTGTG | FT, BclI |
| PP520 | CACCCTGGTTTAAACACTGTTTGCCTGCCAAGCTG | FT, PmeI |
| PP521 | GGCAGGTGATCATGATATGCACCAGGGTGGCGCCA | FT, BclI |
a
Introduced restriction endonuclease sites are in boldface, and the start and mutated nonsense stop codons are underlined.