FIG. 6.

Treatment of epithelial cells with heparinase I has no effect on FIX-mediated Ad31 binding and infection. Ad31 virions were preincubated with or without physiological concentrations of FIX and then allowed to bind to A549 cells (A) or HCE cells (B) or to infect A549 or HCE cells (C) pretreated with increasing concentrations of heparinase I. In binding assays, cell-associated radioactivity was measured with a scintillation counter, whereas in infection assays, infectivity was quantified by fluorescence microscopy.