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Impact of mutating the first and second RXXR motifs on HBeAg production by a genotype B clone. (A) Amino acid sequences of clone B2 and its site-directed mutants, with the four RXXR motifs numbered. Cleavage efficiency in transfected Huh7 cells is indicated by the size of the triangle. (B) IP-Western blot analysis of HBeAg secreted from Huh7 cells. The numbers shown to the right correspond to the RXXR motifs shown in panel A. Mock, mock transfected.
