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. 2009 Feb 4;83(8):3604–3616. doi: 10.1128/JVI.01778-08

FIG. 10.

FIG. 10.

IE2 activation of the CMV promoter is enhanced by siPML and siUBC9 but inhibited by MG132. (A) siRNAs against pml and sumo1 genes were transfected into Vero E6 cells, followed by vAcIE2+vAcL cotransduction. A luciferase assay detected enhanced effects from both siRNAs on IE2 trans-activation of the CMV promoter. siGFP (against egfp) was used as a negative control, while siLUC (against luciferase) was a positive control for siRNA efficiency. Data are presented as percentages of RLU in the absence of siRNA (n = 4). * and **, P < 0.05 and P < 0.001, respectively, compared with the no-siRNA sample (t test). (B) MG132, which blocks the proteasome degradation pathway, had a negative effect on IE2 trans-activation. This result suggested that the ubiquitin ligase activity of IE2 RING domain plays an active role in CMV promoter activation. MG132 did not affect luciferase expression in the control groups (inset).