FIG. 3.

Interaction of IE2 with the CMV promoter. (A) A series of deletion fragments of the CMV promoter were cloned into the pGL-3 luciferase reporter vector. SRE, serum response element. (B) The resulting luciferase expressing vectors were transfected into Vero E6 cells, followed by mock, wt, or vAcIE2 transduction at an MOI of 50. The graph shows the relative luciferase activity from each sample, with CMV-1 set as 100% activity. We calculated the RPS of each promoter for each deletion clone and the ability of IE2 to activate each clone as IE2 activation ratios, calculated as the luciferase level with vAcIE2 transduction/luciferase level with wt transduction.