FIG. 2.

Moderate loss of CFSE in adoptively transferred BCG-immune CD4 T-cell populations. A total of 3 × 10⁷ BCG- or sham-immune spleen leukocytes were transferred intravenously into naïve B6 female mice, which were challenged 4 h later with VV. At 6 days postinfection splenocytes were harvested and analyzed for CD4, CD8, and expression of CFSE. The cells were gated on CD4 or CD8 and plotted against IFN-γ (y axis) and CFSE (x axis). This was part of a larger experiment that included stimulation of intracellular IFN-γ production in vitro, but these plots are intended to show just cell number and are of the nonstimulated controls, so they all are IFN-γ-negative.