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. 2009 Jan 21;83(8):3657–3667. doi: 10.1128/JVI.01966-08

FIG. 1.

FIG. 1.

(A) Detection of PrPSc by highly sensitive WB analysis in the brain stems and nasal mucosae of three scrapie-positive sheep. Lanes 1 to 4, brain stem, MNC, VNC, and NS, respectively, of the three scrapie-positive sheep; lane C−, MNC of a scrapie-negative sample. The amount of tissue equivalents per lane is indicated in milligrams. All samples were proteinase K digested. (B) WB of a positive brain stem sample (identification number, 125704/1/4/06) serially diluted in negative MNC mucosa: 1 × 10−1 (lane 1), 1 × 10−2 (lane 2), 1 × 10−3 (lane 3), 1 × 10−4 (lane 4), 1 × 10−5 (lane 5), and 1 × 10−6 (lane 6). mw, molecular mass. The amount of tissue equivalents per lane is indicated in milligrams. All samples were proteinase K digested. (C) Graphical representation of the relative amounts of PrPSc signal intensity found in MNC, VNC, and NS in relation to brains of three scrapie-positive sheep.