FIG. 1.

Effect of SINV infection and IFN-γ treatment on Stat-1 Tyr₇₀₁ phosphorylation in CSM14.1 cells. (A) Differentiated CSM14.1 cells were infected with SINV, and levels of Stat-1 and phosphorylated (Tyr₇₀₁) Stat-1 were assessed by immunoblotting in cell lysates collected before (0 h) and 2 to 72 h after infection. (B) Infected cells were treated or not treated with 100 U IFN-γ 24 h after infection. (C) Uninfected and infected cells were treated or not treated with IFN-γ 24 h after infection and analyzed 2 to 72 h after treatment. M, mock infected; T, uninfected, IFN-γ treated; I, SINV infected, untreated; I/T, SINV infected, IFN-γ treated.