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. 2009 Feb 4;83(8):3834–3842. doi: 10.1128/JVI.01773-08

FIG. 3.

FIG. 3.

Expression of IFN-λ receptor in macrophages. (A) RT-PCR analysis of IFN-λ receptor gene transcripts in macrophages. Total cellular RNA was extracted from negative control cells (MRC-5 and BB19) and macrophages derived from monocytes from five different donors. Sizes are estimated from a DNA ladder (100-bp fragments) subjected to coelectrophoresis as markers. RT-PCR-amplified products as indicated were visualized on a 1.5% agarose gel. (B) Flow cytometry analysis of IFN-λ receptor (IL-10Rβ) expression in macrophages. IL-10Rβ expression was detected by incubating macrophages with antibody (Ab) directed against IL-10Rβ. Nonspecific goat IgG served as a negative control.