FIG. 5.

Transient expression of CycT1 in undifferentiated monocytes cannot rescue Tat transactivation of LTR. (A) Undetectable CycT1 protein expression in freshly isolated monocytes from three independent donors. Hut/CCR5 cells were used as a positive control. (B) Primary monocytes were nucleofected with pLTR-luc and pTat in the presence or absence of pCycT1. Nucleofected cells were lysed for the detection of luciferase activity at 48 h posttransfection. cps, counts per second. All data are means ± standard deviations. One representative experiment out of three is shown. (C) Detection of CycT1 protein expression. Cells were nucleofected as described for panel B. CycT1 protein expression in nucleofected cells was detected at 48 h posttransfection by immunoblotting. GAPDH was used as a loading control. Relative CycT1 protein levels are shown below each band (normalized to GAPDH and relative to endogenous CycT1 level in Hut/CCR5 cells).