FIG. 7.

Monocyte-differentiated macrophages induce CycT1 protein expression and support efficient HIV-1 infection. (A) Whole-cell lysates of freshly isolated monocytes or monocyte-differentiated macrophages were prepared at the indicated culture times and subjected to immunoblotting. Antibodies to CycT1, total CDK9, phospho-CDK9 (Thr186), and GAPDH were used separately. GAPDH was used as a loading control. Representative results from four different donors are shown. (B) Monocyte-differentiated macrophages support HIV-1 infection. HIV-1 infection of freshly isolated monocytes (day 0) or monocytes differentiated for 1, 3, and 6 days were assessed. Cells were infected with HIV-Luc/VSV-G (MOI, 1). Infected cells were lysed at 3 dpi for the detection of luciferase activity. cps, counts per second. All data are means ± standard deviations. One representative experiment out of three is shown.