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. 2009 Feb 11;83(8):3647–3656. doi: 10.1128/JVI.02028-08

FIG. 5.

FIG. 5.

Induction of apoptosis in RAW264.7 cells is dependent on virus replication. (A) DNA laddering in RAW264.7 cells infected with UV-treated MNV-1 virions. The results of electrophoresis of extracted DNA from UV-treated or nontreated MNV-1-infected RAW264.7 cells are shown and represent MNV-1 infection at 16 h p.i. (lane 4), UV-treated MNV-1-infected RAW264.7 cells (lane 3), mock-infected RAW264.7 cells (lane 2), and a DNA marker (lane 1). (B) Effect of CHX on MNV-1 replication in infected RAW264.7 cells. Mock- and MNV-1-infected RAW264.7 cells were treated with no CHX (lanes 1 and 4), with 0.5 μg of CHX/ml (lanes 2 and 5), with 1 μg of CHX/ml (lanes 3 and 6), and with staurosporine (ss; lane 7). Equivalent amounts of protein collected at 16 h p.i. were loaded in each gel and detected with α-PCNA antibody in infected and mock-infected cultures with or without CHX (lanes 1 to 7). The virus polymerase (NS7) was observed in virus-infected cells without CHX in the growth medium (lane 1). Cleaved caspase-9 was observed in MNV-1-infected cells without CHX (lane 1) and in RAW264.7 cells in the presence of an apoptosis inductor, staurosporine (ss) (lane 7).