FIG. 6.

Downregulation of BIRC5 (survivin) over time in MNV-1-infected RAW264.7 cells. The levels of BIRC5 and survivin, as well as the level of genomic MNV-1 RNA, are shown. Survivin transcription in MNV-1-infected cells compared to mock-infected cells (24 h p.i.) was normalized to the level of ß-actin transcription. MNV-1 genomic RNA detection by quantitative real-time PCR is plotted in comparison to the decreasing levels of BIRC5. Survivin was detected by Western blot analyses of infected cells corresponding to the same time points of MNV-1 infection. Equivalent protein amounts were loaded in each gel and detected with anti-PCNA antibody.