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. 2009 Feb 2;29(8):1999–2010. doi: 10.1128/MCB.00982-08

FIG. 3.

FIG. 3.

Plk3 mRNA half-life in WT and ZFP36L1-and ZFP36L2-deficient fibroblasts. The half-lives of Plk3 mRNA in paired fibroblast cell lines deficient in either ZFP36L1 or ZFP36L2 were compared. Serum-starved fibroblasts were stimulated for 90 min with FBS and treated with actinomycin D (Act D) for the indicated times. (A and C) Representative Northern blots are shown for Plk3 mRNA decay and GAPDH expression for WT and ZFP36L1-KO (A) and WT and ZFP36L2-KO (C) fibroblasts. (B) The mean half-lives of Plk3 mRNA normalized for GAPDH mRNA expression, when calculated from three different paired fibroblast cell lines, were 55 and 42 min for WT and ZFP36L1-deficient fibroblasts, respectively. (D) The half-lives of Plk3 mRNA from three different paired cell lines were 44 and 34 min for WT and ZFP36L2 KO cells, respectively. The black and white squares represent the WT and KO cell lines, respectively. Error bars represent the SD derived from data for three to four separate experiments, with the Northern blots being quantitated by phosphorimager analysis and normalized for GAPDH mRNA levels.