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. 2009 Feb 17;29(8):2322–2334. doi: 10.1128/MCB.01543-08

FIG. 1.

FIG. 1.

Pax6 regulates the PC2 and 7B2 genes. (a) Real-time quantitative RT-PCR from RNA obtained from InR1G9 cells transfected with a scrambled (Sc) or Pax6 siRNA (siPax6) for 48 h. The analysis was performed with the LightCycler software (Roche Applied Science). The results were corrected for TBP mRNA levels. The relative mRNA levels are presented as the means ± standard errors of the means (SEM) of at least three independent experiments. *, P < 0.05; **, P < 0.01. Left corner, Western blot analyses of Pax6 and TFIIE from nuclear extracts from InR1G9 cells transfected with scrambled or Pax6 siRNA for 48 h. Ab, antibody. (b) Western blot analyses of PC2, 7B2, and TFIIE from total extracts from InR1G9 cells transfected with scrambled or Pax6 siRNA for 48 h. (c) Real-time quantitative RT-PCR analysis of RNA obtained from the A5 (pRC-CMV-expressing) and C4 (Pax6-DN306-expressing) clones. The analysis was performed with the LightCycler software (Roche Applied Science). Left panel, measurement of PC2 mRNA, right panel, measurement of 7B2 mRNA. The results were corrected for actin mRNA levels. The relative mRNA levels are presented as the means ± SEM for at least three independent experiments. *, P < 0.05; **, P < 0.01. (d) Western blot analyses of PC2, 7B2, and TFIIE from total cell extracts from A5 and C4 clones.