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. 2009 Feb 17;29(8):2322–2334. doi: 10.1128/MCB.01543-08

FIG. 3.

FIG. 3.

Activation of the PC2 and 7B2 promoters by ectopic expression of Pax6, cMaf, MafB, and Beta2/NeuroD1 in a heterologous cell line. (a) BHK21 cells were transfected with the −730 WT mouse PC2 promoter (open bars), the empty reporter vector (pGL2-Basic), a site-directed mutation-containing form of the large-Maf (xMaf) or Beta2/NeuroD1 (xBeta2) site, or both sites (solid bars) fused to the Luc reporter gene along with the Pax6, cMaf, MafB, Beta2/NeuroD1 (Beta2), and E47 cDNAs and the Egr1 cDNA used as a positive control. Results represent relative Luc and PAP activities over the basal level for each construct. *, P < 0.05; **, P < 0.01 compared to the transfection of the promoter with pSG5. (b) BHK21 cells were transfected with the −1228 WT mouse 7B2 promoter (open bars), the empty reporter vector (pGL2-Basic), or a site-directed mutation-containing form of the large-Maf (xMaf), Beta2/NeuroD1 (xBeta2), Pax6 (xPax6-1, or xPax6-2) site, alone or combination (solid bars), fused to the Luc reporter together with the Pax6, cMaf, MafB, Beta2/NeuroD1, and E47 cDNAs. Results represent relative Luc and PAP activities over the basal level for each construct. Values are the means ± the standard errors of the means from at least three separate experiments, each performed twice. *, P < 0.05; **, P < 0.01 (compared to the transfection of the promoter with pSG5); #, P < 0.05 (compared to the WT 7B2 plasmid together with the respective transcription factor).