TABLE 2.

Comparison of death rates in single- and double-knockdown embryos treated with tbx5 and mef2c morpholinos^a

Expt and morpholino dose	No. of embryos injected	Rate (%) by 48 hpf
Single knockdown
None (wild type)	2,304	3b
5 ng tbx5	140	5b
9 ng tbx5	107	14b
16.5 ng tbx5	88	11b
3 ng mef2c	109	8b
7.5 ng mef2c	136	10b
8.3 ng mef2c	43	4b
Double knockdown
3 ng each (6 ng total)	30	37b
4 ng each (8 ng total)	332	65b
5 ng each (10 ng total)	297	79b
Single and double knockdown at lower doses
1.24 ng tbx5	533	21c
1.86 ng tbx5 mis	302	1c
1.86 ng mef2c	245	6c
1.86 ng mef2c mis	233	3c
1.24 ng tbx5 + 1.86 ng mef2c	420	52c
1.24 ng tbx5 + 1.86 ng mef2c mis	358	19c
1.24 ng tbx5 mis + 1.86 ng mef2c	381	21c
1.24 ng tbx5 mis + 1.86 ng mef2c mis	285	1c

^aOne- or two-cell zebrafish embryos were injected with morpholino, and mortality by 48 hpf was recorded.

^bDeath rate.

^cMorphant rate.