TABLE 2.
Expt and morpholino dose | No. of embryos injected | Rate (%) by 48 hpf |
---|---|---|
Single knockdown | ||
None (wild type) | 2,304 | 3b |
5 ng tbx5 | 140 | 5b |
9 ng tbx5 | 107 | 14b |
16.5 ng tbx5 | 88 | 11b |
3 ng mef2c | 109 | 8b |
7.5 ng mef2c | 136 | 10b |
8.3 ng mef2c | 43 | 4b |
Double knockdown | ||
3 ng each (6 ng total) | 30 | 37b |
4 ng each (8 ng total) | 332 | 65b |
5 ng each (10 ng total) | 297 | 79b |
Single and double knockdown at lower doses | ||
1.24 ng tbx5 | 533 | 21c |
1.86 ng tbx5 mis | 302 | 1c |
1.86 ng mef2c | 245 | 6c |
1.86 ng mef2c mis | 233 | 3c |
1.24 ng tbx5 + 1.86 ng mef2c | 420 | 52c |
1.24 ng tbx5 + 1.86 ng mef2c mis | 358 | 19c |
1.24 ng tbx5 mis + 1.86 ng mef2c | 381 | 21c |
1.24 ng tbx5 mis + 1.86 ng mef2c mis | 285 | 1c |
One- or two-cell zebrafish embryos were injected with morpholino, and mortality by 48 hpf was recorded.
Death rate.
Morphant rate.