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. 2009 Feb 17;29(8):2278–2295. doi: 10.1128/MCB.01768-08

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FIG. 9. — DNA synthesis decreases during S phase, and γ-H2AX foci increase after conditional knockout of PR-Set7 in ES cells. (A and B) Immunofluorescence analyses using untreated PR-Set7^flox/⁻; Cre^ERT cells (−4OHT) or those exposed to 4-OHT for 48 h [+4OHT (48 h)] as indicated at the top. Colocalization of antibody specific to γ-H2AX with either EdU or antibody specific to phospho-ATM is shown (merge). Phosphorylation of γ-H2AX at serine 139 and of the ATM kinase at serine 1981 occurs in response to DNA damage. (C) The percentage of PR-Set7^flox/⁻; Cre^ERT cells exhibiting the indicated number of foci of colocalized γ-H2AX and phospho-ATM as a function of 4-OHT treatment for the number of hours shown at the bottom. The number of colocalized foci increased dramatically after knockout of PR-Set7.