Skip to main content
. 2009 Feb 17;29(8):2118–2128. doi: 10.1128/MCB.01503-08

FIG. 6.

FIG. 6.

Requirements of the Retromer complex for Wnt signaling during early Xenopus development. (A) Embryos were unilaterally injected with Vps35 MO (20 ng) alone or together with XWntless-HA mRNA (333 pg), with LacZ mRNA. At neurula stage, LacZ-stained embryos were subjected to in situ hybridization with Twist, Sox2, XAG, Otx2, En2, Krox20, HoxB9, Msx1, Pax6, or Wnt4 probe. Arrows indicate the injected sides. Dashed lines indicate the midlines of the injected embryos. (B) Embryos were unilaterally injected with Vps35 MO (20 ng) alone or together with β-catenin mRNA (500 pg) or XDsh mRNA (500 pg), with LacZ mRNA. At neurula stage, LacZ-stained embryos were subjected to in situ hybridization with Twist, Sox2, or En2 probe. Arrows indicate the injected sides. Dashed lines indicate the midlines of the treated embryos. (C) Two-cell-stage embryos were bilaterally injected at animal pole and raised until stage 12. Whole embryos were harvested and subjected to Western blotting for anti-phospho-Smad1 and anti-phospho-ERK1/2. Anti-Smad1, anti-ERK, and antiactin were used as loading controls. p, phospho. (D) Secretions of ectopic XWnt1, XWnt3a, XWnt5a, XWnt8, and XWnt11 were measured by Western blotting. Vps35 MO and XWntless-HA mRNA were coinjected with myc-tagged Wnts as indicated. W1, XWnt1; W3a, XWnt3a; W5a, XWnt5a; W8, XWnt8; W11, XWnt11; +, present; −, absent; WLS, XWntless.