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. 2009 Feb 17;29(8):2129–2138. doi: 10.1128/MCB.01644-08

FIG. 1.

FIG. 1.

Expression of MFng in Ngn3+ proendocrine cells of the developing pancreas. E10.5 (A to C), E9.5 (D to F), and E14.5 (G to L) wild-type pancreases were analyzed by in situ hybridization. (A to C) MFng (black in panel A), Dll1 (black in panel B), and Ngn3 (black in panel C) are expressed in scattered cells within the dorsal pancreas at E10.5. (D to F) MFng (green pseudocolor in panel D) is coexpressed with Ngn3 (red immunohistochemical staining in panel D) at E9.5, whereas the expression of LFng (E) and RFng (F) is not detected. (G, H, J, and K) Dll1 (green pseudocolor in panels G and J) and MFng (green pseudocolor in panels H and K) are transiently expressed in proendocrine cells expressing Ngn3 (red immunohistochemical staining in panels G and H) but downregulated in differentiated endocrine cells expressing Isl1 (red immunohistochemical staining in panels J and K). (I and L) LFng (green pseudocolor in panel I) is coexpressed with Ptf1a (red immunohistochemical staining in panel I) in exocrine acinar cells, whereas RFng (L) is not expressed in pancreatic epithelia at E14.5. Broken lines (A to F and L) delimit pancreatic epithelia. Open arrowheads mark cells positive for in situ hybridization but negative for Ngn3, Isl1, or Ptf1a. Closed arrowheads mark cells positive for in situ hybridization and Ngn3 or Ptf1a. Arrows mark cells negative for in situ hybridization but positive for Ngn3 or Isl1. a, acinar exocrine cells; e, pancreatic epithelia; m, mesenchyme.