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. 2009 Feb 17;29(8):2129–2138. doi: 10.1128/MCB.01644-08

FIG. 3.

FIG. 3.

Normal endocrine cell differentiation and β-cell function in Ipf1-MFng transgenic mice. (A to E′) Expression of progenitor and differentiation markers in the pancreases of wild-type (WT) (A to E) and Ipf1-MFng (A′ to E′) mice at E10.5 (A to B′) and E14.5 (C to E′). (A to B′) At E10.5, the forced expression of MFng (compare panels A and A′) does not affect the expression of Dll1 (blue pseudocolor in panels B and B′), Ngn3 (red immunohistochemical staining in panels B and B′), or glucagon (green immunohistochemical staining in panels B and B′). (C to E′) At E14.5, Ipf1-MFng pancreases show normal expression of Ngn3 and glucagon (red and green immunohistochemical staining, respectively, in panels D and D′) and insulin and ghrelin (green and red immunohistochemical staining, respectively, in panels E and E′). (F) Quantification of the epithelial areas occupied by Ngn3+ cells and glucagon-positive α-cells at E10.5. Values are presented relative to wild-type values and are averages ± SEM (n = 3). (G) The clearance of glucose from the blood after the intraperitoneal (i.p.) injection of Ipf1-MFng-lacZ transgenic (n = 7) and wild-type (n = 5) mice and Ipf1-MFng transgenic (n = 4) and wild-type (n = 4) mice with glucose was normal. Values are shown with SEM. Broken lines in panel A delimit pancreatic epithelia.